Tracking clonal evolution during treatment in ovarian cancer using cell-free DNA.

ABSTRACT: Emergence of drug resistance is the main cause of therapeutic failure in patients with high-grade serous ovarian cancer (HGSOC)¹. To study drug resistance in patients, we developed CloneSeq-SV, which combines single-cell whole-genome sequencing² with targeted deep sequencing of clone-specific genomic structural variants in time-series cell-free DNA. CloneSeq-SV exploits tumour clone-specific structural variants as highly sensitive endogenous cell-free DNA markers, enabling the relative abundance measurements and evolutionary analysis of co-existing clonal populations over the therapeutic time course. Here, using this approach, we studied 18 patients with HGSOC over a multi-year period from diagnosis to recurrence and showed that drug resistance typically arose from se

SUBMITTER: Williams MJ 

PROVIDER: S-EPMC12629990 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature