A transcription termination mechanism for maintaining homogeneous protein expression.

ABSTRACT: Premature transcription termination is a critical mechanism for repressing harmful, unproductive transcription. In Saccharomyces cerevisiae, premature termination by the Nrd1-Nab3-Sen1 (NNS) complex also triggers decay of mRNAs induced to counteract stress. However, why mRNAs might be upregulated and simultaneously targeted for degradation remains unclear. Our work reveals a novel role for NNS as a gene expression noise suppression system. Single-cell analyses demonstrate that targeted disruption of Nab3 binding to the highly conserved mitochondrial transporter PIC2 not only alters its mRNA stability and protein levels but also dramatically increases cell-to-cell variability in Pic2 protein concentration. This specific perturbation in the regulation of a low-abundant transcript has substan

SUBMITTER: Esteban-Serna S 

PROVIDER: S-EPMC12630142 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature