Investigating the effects of membrane curvature inducing proteins on lipid droplets in Saccharomyces cerevisiae.

ABSTRACT:

Background

Reticulons (Rtn1p, Rtn2p) and Yop1p are proteins shaping the endoplasmic reticulum (ER) membrane curvature their function being to a large degree responsible for molding the membrane curvature in the tubular ER area. Lipid biosynthesis mainly takes place in the tubular parts of the ER and lipid droplets are formed in the tubular ER membrane. Nucleation constitutes the rate-limiting step in lipid droplet formation and is modulated by membrane curvature; specifically increasing membrane curvature lowers the activation energy required for lipid lens nucleation. The aim of this work was to investigate the effects, modifying the ER membrane curvature would have on lipid droplets in Saccharomyces cerevisiae.

Methods and results

We created and screened strains overexpres

SUBMITTER: Niemela LRK 

PROVIDER: S-EPMC12630273 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature