Farnesyltransferase inhibitors decrease matrix-vesicle-mediated mineralization in SaOS-2 cells.

ABSTRACT:

Background

Farnesyltransferase inhibitors (FTIs) were developed for preventing the prenylation of aberrant Ras in human cancer. Furthermore, prenylation is involved in multiple biological processes and plays a putative role in signalling pathways such as the Ras-mitogen-activated-protein kinase (MAPK) pathway. Matrix-vesicle-mediated mineralization (MVM) is the first step in the development of eukaryotic mineralized tissue. In this study, we evaluated the effect of FTIs on MVM in the osteosarcoma cell line SaOS-2 and elucidated the role of farnesylation in this process.

Methods

SaOS-2 cells were treated with the FTIs Lonafarnib and Tipifarnib. Mineralization was assessed using Alizarin Red S staining. Enzyme assays were conducted to measure alkaline phosphatase (ALP) activit

SUBMITTER: Burgel T 

PROVIDER: S-EPMC12630319 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature