MLO-mediated Ca²⁺ influx regulates root hair tip growth in Arabidopsis.

ABSTRACT: Root hair tip-growth involves coordinated Ca²⁺ and ROS signaling to promote growth while maintaining tip integrity. MILDEW RESISTANCE LOCUS-O (MLO) proteins act downstream of FERONIA (FER) receptor-like kinases in pollen tubes and synergids to regulate calcium dynamics. This study uses a constitutively active MLO (faNTA) to identify a new role for the FER/MLO signaling module in regulating [Ca²⁺]_cyt oscillations in growing root hairs. faNTA was used as a tool to manipulate Ca²⁺ influx in fer mutants. Light sheet fluorescence imaging was used to image the reporter R-GECO1 to observe [Ca²⁺]_cyt dynamics during root hair elongation in various genotypes. We show that faNTA is sufficient to restore normal root hair development, [C

SUBMITTER: Ogawa ST 

PROVIDER: S-EPMC12630461 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature