Three-dimensional multicellular layer structure: an advanced in vitro model for studying inflammatory bowel diseases.

ABSTRACT: Inflammatory Bowel Disease (IBD) is a group of complex and debilitating gastrointestinal disorders characterised by chronic inflammation of the intestinal mucosa. This condition is multifactorial, involving genetic predispositions, environmental influences, dysregulated immune responses, and impaired epithelial barrier function. Researchers have already proposed the use of Extracellular Vesicles (EVs) as a therapeutic approach for inflammatory diseases. EVs are lipid bilayer-delimited nanoparticles secreted by cells that possess anti-inflammatory and pro-regenerative properties. We aim to develop a three-dimensional (3D) multilayer structure (MLS) model that mimics the physiological complexity of the intestinal mucosa, serving as a ready-to-use primary platform for drug testing. MLS, built

SUBMITTER: Zaramella A 

PROVIDER: S-EPMC12630607 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature