Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy.

ABSTRACT: Recent advances in fluorescence nanoscopy have pushed resolution to the 1-10 nm range, enabling the direct visualization of individual molecules even in crowded biological environments. Achieving this level of precision requires rigorous sample drift control. Techniques such as MINFLUX and RASTMIN, which rely on keeping the sample fixed within an excitation pattern, demand active drift correction to achieve their theoretical nanometer-scale resolution limits. Here, we present an active stabilization system for super-resolution microscopy that delivers sub-nm precision for hours. Featuring a simple optical design, the system can be added as a separate module to any fluorescence microscope. We also provide an open-source control software including a user-friendly graphical interface readily

SUBMITTER: Edorna F 

PROVIDER: S-EPMC12630732 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature