GLP-1RA in JBMMSCs promoted osteogenic differentiation via modulating CREB and BRD4 signaling mediated proliferation and stemness.

ABSTRACT: This study aims to examine the molecular mechanisms underlying the curative effects of jaw bone marrow mesenchymal stem cells (JBMMSCs) on rat mandibular osteoporosis following semaglutide administration. We acquired data from rats with osteoporosis via the GEO database for bioinformatics analysis. JBMMSCs (Jaw Bone Marrow Mesenchymal Stem Cells) were then isolated from rat mandibles and characterized via flow cytometry. To evaluate the therapeutic effect of semaglutide on osteoporosis following JBMMSC intervention in osteoporotic rat models, we employed Haematoxylin and eosin (HE) staining. In vitro, the ideal semaglutide concentration for JBMMSC osteogenic induction was established using CCK-8 viability, colony formation, and scratch migration assays. JBMMSCs were subsequently injected i

SUBMITTER: Liu H 

PROVIDER: S-EPMC12630770 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature