Engineering a new tripartite split-ccGFP system from Corynactis californica for detecting protein-protein interactions.

ABSTRACT: Protein-protein interactions (PPIs) are critical to a range of biological processes and, consequently, aberrant interactions are implicated in many disorders. The study of the complex networks of PPIs promises to elucidate undiscovered roles in cellular processes and the mechanisms of disease. To accomplish this, tools to effectively sense PPIs are necessary. Effective PPI sensors must rapidly detect interactions in real-time with high sensitivity without perturbing the proteins of interest (POIs) under study. Split fluorescent proteins have previously been used to successfully monitor PPIs, in part due to the small size of the tags. Here, we developed an optimized tripartite split GFP system based on Corynactis californica GFP (ccGFP) to detect PPIs in vitro. In this sensor system, ccGFP

SUBMITTER: Groseclose TM 

PROVIDER: S-EPMC12630887 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature