Dynamic proteome profiling of differentiating human embryonic stem cells towards cardiomyocytes.

ABSTRACT: The heart is the first functional organ to develop during embryogenesis, yet its cellular and molecular mechanisms remain incompletely understood. Proteomic studies of in vitro cardiomyogenesis have provided valuable insights, but most have focused on late-stage processes leading to cardiomyocyte specification. In this study, we profiled the proteome of differentiating cells at the mesendoderm (ME), cardiac mesoderm (CME), cardiac progenitor cell (CPC), and cardiomyocyte (C) stages using in-depth quantitative analysis via multiplexed tandem mass tag-based mass spectrometry. Unsupervised clustering of 4417 differentially expressed proteins revealed six main clusters corresponding to key biological processes at each differentiation stage. Transitional-specific proteins (TSPs), identified thr

SUBMITTER: Meyfour A 

PROVIDER: S-EPMC12630888 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature