In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy.

ABSTRACT: Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the most prevalent genetic kidney disorder. Animal preclinical studies are one of the main tools to study this disease, often through either 2D histology imaging for high-resolution analysis or CT or MRI for full kidney segmentation. As an alternative to these modalities, we propose the use of Light Sheet Fluorescence Microscopy (LSFM) for high-resolution 3D imaging of healthy and ADPKD-induced mouse kidneys, enabling a detailed volumetric morphological analysis of the disease's effects. In a mouse ADPKD model, ex vivo imaging of the kidneys was performed through LSFM, after which a combination of machine learning and other processing techniques allowed us to perform an in-depth image analysis. This includes the segmentation of key st

SUBMITTER: Delgado-Rodriguez P 

PROVIDER: S-EPMC12630910 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature