Production of offspring via the transplantation of frozen germ cells from Tokyo bitterling, a fish on the brink of extinction.

ABSTRACT: Many freshwater fish face extinctions due to habitat destruction and overfishing. Bitterlings, which lay their eggs in the gill chambers of freshwater mussels, are in steep decline worldwide, along with mussels, owing to the concreting and siltation of riverbeds. In particular, Tokyo bitterling is listed as "endangered" on the Red List and is on the verge of extinction. Although in situ and ex situ conservation efforts are underway, their effectiveness is limited. Here, we developed a method for long-term preservation of genetic resources by combining germ cell cryopreservation and transplantation. We transplanted Tokyo bitterling germ cells stored in liquid nitrogen into oily bitterling recipients lacking endogenous germ cells due to dnd knockdown, and the recipients matured and repeatedl

SUBMITTER: Yamakawa K 

PROVIDER: S-EPMC12630933 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature