Development of an Electrochemical Immunosensor for Detecting Coagulation Factor Xa and Perspectives in Monitoring Direct Oral Anticoagulant Therapy.

ABSTRACT: This study aims to develop a point-of-care (POC) electrochemical immunosensor to monitor the anticoagulant activity of direct oral anticoagulants (DOACs) by detecting and distinguishing between the inactive (FX) and active (FXa) forms of Factor X. DOACs have transformed the management of thromboembolic disorders by selectively inhibiting FXa. Common monitoring methods are either insufficiently sensitive to DOAC levels or not widely accessible. This study demonstrates the development of a graphene-based electrochemical immunosensor modified by gold nanostructures and functionalized with anti-FX antibodies. Electrode modifications were characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and field-emission scanning electron microscopy (FESEM), showing enhanced elec

SUBMITTER: Meireles MR 

PROVIDER: S-EPMC12631654 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature