Establishing ¹⁸O-Labeled Inositol Phosphates for Quantitative Capillary Electrophoresis-Mass Spectrometry: Fragmentation Pathways and Comparison with ¹³C-Labeled Analogs.

ABSTRACT: Capillary electrophoresis mass spectrometry (CE-MS) allows for the rapid and accurate quantitative analysis of inositol phosphates (InsPs) and inositol pyrophosphates (PP-InsPs). The recent discovery of new InsPs and PP-InsPs isomers in plants and mammals necessitates new heavy isotope references for quantitative analysis of complex cellular extracts. Here, we evaluate ¹⁸O-labeled InsPs and PP-InsPs as alternatives to ¹³C labeled internal standards for quantitation by CE-MS. In contrast to ¹³C labels, the ¹⁸O labels are introduced at the end of a synthetic campaign and not at the beginning, rendering ¹⁸O much more accessible and affordable as a label. A series of ¹⁸O-labeled InsPs and PP-InsPs with different numbers and posi

SUBMITTER: Liu G 

PROVIDER: S-EPMC12631729 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature