Scatter-Free UV-Visible Spectroscopy for Accurate and Precise RNA Quantification in Complex RNA Nanoparticle Formulations.

ABSTRACT: Ribonucleic acid (RNA)-based drugs showed the potential for treating wide range of diseases. Their successful clinical use depends on developing complex nanoparticle (NP) formulations made from diverse biomaterials. Accurately quantifying total RNA concentration in complex formulations is challenging, often requiring expensive, low-throughput methods or fluorescence-based assays like RiboGreen that rely on effective NP disruption. This study evaluates scatter-free absorption spectroscopy (SFAS), a UV/Visible method that removes light scattering from NP components and enables accurate total RNA quantification in intact NPs. To validate SFAS, we employed diverse RNA formulations, including lipid NPs, polymer and dendrimer hybrid lipid NPs, and cyclodextrin nanocomplexes, which exhibit physic

SUBMITTER: Lopez Espinar A 

PROVIDER: S-EPMC12631730 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature