Innovative Application of a Multifunctional Sucrose-Gelatin Hydrogel Matrix in Desorption Electrospray Ionization-Mass Spectrometry Imaging.

ABSTRACT: Desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) is among the most powerful techniques for visualizing the spatial distribution of small organic molecules, particularly lipids, on tissue surfaces. Conventional DESI-MSI analysis typically involves sectioning fresh-frozen tissues or, less commonly, embedding samples in matrices specifically formulated to preserve the tissue integrity for multifunctional analyses. In this study, we present an optimized sucrose-gelatin hydrogel matrix compatible with DESI-MSI, using mouse brain tissue as a model system. The method involves low-temperature embedding of frozen specimens into the hydrogel matrix, followed by snap-freezing at -160 °C. This matrix formulation ensures minimal background interference and prevents metabolite del

SUBMITTER: Ziaco M 

PROVIDER: S-EPMC12631731 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature