Clinical application of metagenomic next-generation sequencing in the diagnosis of severe pneumonia pathogens.

ABSTRACT:

Background

Severe pneumonia is a significant cause of mortality among ICU patients. Metagenomic next-generation sequencing (mNGS) is an advanced, comprehensive, unbiased diagnostic tool for pathogen identification in infectious diseases. This study aimed to evaluate the clinical efficacy of mNGS for diagnosing severe pneumonia.

Methods

This study retrospectively analyzed 323 patients with suspected severe pneumonia admitted to the intensive care unit (ICU) of Wuhan University Renmin Hospital between January 2022 and December 2023. Bronchoalveolar lavage fluid (BALF) samples were collected from all 323 patients, and blood samples were obtained from 80 patients. Both mNGS and conventional microbial testing (CMT) were performed on the collected BALF and blood samples to analyze

SUBMITTER: Huang R 

PROVIDER: S-EPMC12631761 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature