Optimization of the HS-GC/MS technique for urine metabolomic profiling.

ABSTRACT:

Background

Headspace gas chromatography-mass spectrometry (HS GC-MS) traditionally has been applied to analyze samples with a high content of volatile components, such as stool samples. Nevertheless, other types of samples-for example, urine-may also contain volatile compounds and serve as valuable sources of diagnostic information. However, the content of volatile components in urine is considerably lower than in stool samples, necessitating modification of the HS GC/MS method. Such optimization could be particularly valuable for patients with inflammatory bowel disease (IBD), for whom providing a stool sample can sometimes be challenging. The aim of this work was to optimize a method for assessing volatile components in urine samples.

Methods

Urine samples were collected f

SUBMITTER: Zakharzhevskaya NB 

PROVIDER: S-EPMC12631782 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature