Computational Tools and Resources for Long-read Metagenomic Sequencing Using Nanopore and PacBio.

ABSTRACT: In recent years, the field of shotgun metagenomics has witnessed remarkable advancements, primarily driven by the development and refinement of next-generation sequencing technologies, particularly long-read sequencing platforms such as Nanopore and PacBio. These platforms have significantly improved the ability to analyze microbial communities directly from environmental samples, providing valuable information on their composition, function, and dynamics without the need for pure cultivation. These technologies enhance metagenomic data assembly, annotation, and analysis by addressing longer reads, higher error rates, and complex data. In this review, we provide a comprehensive overview of the historical development of long-read metagenomics, highlighting significant landmarks and advancem

SUBMITTER: Zhang 张天缘 T 

PROVIDER: S-EPMC12631790 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature