Neoeriocitrin Targeting Beclin1 Deubiquitination and Autophagy in Osteogenic Differentiation of Human Dental Pulp Stem Cells.

ABSTRACT: Human dental pulp stem cells (hDPSCs) are dental-derived mesenchymal stem cells with robust multipotent differentiation potentials, rendering them promising for bone tissue engineering. However, their differentiation relies on expensive, hard-to-control growth factors. Neoeriocitrin (Neo), a natural flavonoid, promotes cell proliferation and regulates alkaline phosphatase activities. However, Neo's effect on hDPSCs osteogenesis and bone regeneration is unknown. This study investigated Neo's impact on hDPSCs osteogenic differentiation and its mechanisms for bone regeneration. Neo effectively boosted hDPSCs osteogenic differentiation in vitro and facilitated bone regeneration in rat calvarial defects in vivo. Thermal proteome profiling revealed Neo directly binds Beclin1, validated by cellul

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12631872 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature