Centimeter-Scale Self-Assembling Tendon Organoids Drive Tissue Regeneration.

ABSTRACT: As a cell-deficient tissue, the scarcity of endogenous stem cells significantly hampers the regeneration of tissue structure and restoration of motor function following tendon injury. To engineer large-scale transplantable stem cell-derived organoids in vitro would show tremendous potential in regenerative medicine. Here, by optimizing chemical signals and mimicking tendon extracellular matrix, transplantable tendon organoids exceeding 3 cm in human tissue-scale dimensions are ultimately developed. This strategy empowers tendon organoids, with high cellular viability, proliferation, tenogenic phenotype, and remarkable enhancements in extracellular matrix (ECM) production enabled self-assembly. At the single-cell level, the majority cells in tendon organoids successfully achieve precise ten

SUBMITTER: Fang T 

PROVIDER: S-EPMC12631886 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature