Single Mitochondrion Morphology-Function Relationship Analysis Using Fluorescent Probes and Artificial Intelligence.

ABSTRACT: The ability to decode the relationship between mitochondrial morphology and function at the level of individual organelles is central to understanding cellular responses to stress, such as hypoxia. Herein, a comprehensive strategy is presented that integrates tailored fluorescent probes with artificial intelligence (AI) for single mitochondrion analysis. Focus is on three interrelated biomarkers, reactive oxygen species (ROS), viscosity, and mitochondrial membrane potential (MMP), that together form a pathophysiological axis indicative of mitochondrial state under hypoxic stress. A functional probe set is used to image these features simultaneously, including a newly developed dual-cationic probe, MitoVP, which enhances mitochondrial targeting and resolution for viscosity sensing. Mitochon

SUBMITTER: Ding Y 

PROVIDER: S-EPMC12631887 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature