OsNRT1.1B-OsCNGC14/16-Ca²⁺-OsNLP3 Pathway: Phosphorylation-Mediated Maintenance of Nitrogen Homeostasis.

ABSTRACT: Nitrate, a crucial nutrient and signaling molecule, is extensively studied across plants. While the NRT1.1-NLP-centered pathway dominates nitrate signaling in Arabidopsis and rice, however, whether there is functional interaction or co-regulation between the primary nitrate response (PNR) and long-term nitrogen utilization remains unclear. Here, a novel nitrate signaling pathway is identified in rice that works alongside the established ubiquitination-mediated OsNRT1.1B-OsSPX4-OsNLP3 cascade. It is demonstrated that OsCNGC14, OsCNGC16, and OsNRT1.1B form a plasma membrane-localized complex in root tips, mediating nitrate-triggered Ca²⁺ influx. The absence of either OsCNGC14 or OsCNGC16 abolished Ca²⁺ signaling and suppressed PNR. The OsNRT1.1B-OsCNGC14/16 complex acti

SUBMITTER: Wang X 

PROVIDER: S-EPMC12631913 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature