Identifying Novel DNA Adducts in Amphipods and Developing Sample Preparation for Adductomics Using Dispersive Solid-Phase Extraction.

ABSTRACT: The growing use of environmental DNA adductomics for exposure assessment emphasizes the need for improved methods that are adapted to chemically and biologically diverse samples. While liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) has advanced DNA adductome analysis, sample cleanup methods remain challenging, especially in nonmammalian models. Here, we used amphipod Monoporiea affinis, a sentinel species in biological effect studies, as a surrogate crustacean species for the method development. It is particularly suitable because its chitinous tissues and high lipid content present a challenging matrix for DNA extraction. We addressed the following objectives: (i) to propose adduct structures by combining multiple tools for DNA adduct screening and (ii

SUBMITTER: Khan Z 

PROVIDER: S-EPMC12631988 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature