Improving succinic acid production by tuning polysulfides metabolism in Yarrowia lipolytica.

ABSTRACT:

Backgroud

Succinic acid (SA) is a significant C4-dicarboxylic acid with broad applications in the food, chemical, and pharmaceutical industries. In microbial SA production, Yarrowia lipolytica shows great potential. Polysulfides are vital for maintaining redox balance and cellular health in yeast.

Results

In this study, we changed the polysulfides metabolism of Y. lipolytica to enhance SA production. The 3-mercaptopyruvate sulfurtransferase (3-MST) and rhodanese (RHOD) encoding genes were disrupted in Y. lipolytica PGC01003, which led to increased biomass and SA production. In a 3-L scale bioreactor, the mutant strain produced 64.5 g/L SA, representing a 37.8% increase compared with PGC01003. Further investigations indicated that the number of mitochondria was decreased, but

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12632031 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature