Mining expansin-like proteins from rumen microbiota and functional characterization of two anaerobic fungal expansin-like proteins.

ABSTRACT:

Background

Sustainable livestock production is essential for food security and environmental management. Lignocellulosic biomass can be used in animal feed, thereby reducing feed production costs and enhancing sustainability. Expansin-like proteins (ELPs) play essential roles in plant cell wall degradation, yet their functions remain largely underexplored in rumen microbes. The purpose of this study was to investigate the effects of rumen microbial ELPs on lignocellulose degradation.

Results

This study systematically identified 396 ELPs within the rumen microbiota, uncovering remarkable diversity, particularly among anaerobic fungi. Three representative ELPs from Pecoramyces ruminantium F1 (PFLoos_1, PFSWO1_1, PFSWO2_1) were selected for biochemical characterization. While P

SUBMITTER: Dai H 

PROVIDER: S-EPMC12632131 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature