De novo design of a two-step approach targeting Claudin-6 for enhanced drug delivery to solid tumors.

ABSTRACT:

Background

Although antibody-conjugated drugs have achieved success in clinical practice for cancer treatment, challenges remain in developing a highly efficient drug delivery system with specific accumulation in tumors and reduction in side effects. With improved pharmacokinetics, strong covalent bonding and quick binding reactions, a pre-targeting approach via molecular pairs represents an attractive platform for two-step delivery system construction.

Methods

Bioinformatics and immunohistochemistry assays were performed to assess Claudin-6 (CLDN6) as a highly specific tumor target in solid tumors. A phage-displayed library was used to screen and optimize anti-CLDN6 designed ankyrin repeat proteins (DARPins), which were incorporated into a two-step delivery system based on

SUBMITTER: Yan J 

PROVIDER: S-EPMC12632142 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature