<i>Escherichia coli</i> is poised to grow using 5'-deoxynucleosides via MtnR and CRP regulation of DHAP shunt gene expression.

ABSTRACT: The dihydroxyacetone phosphate (DHAP) shunt is a multifunctional pathway for the metabolism of 5'-deoxynucleosides and 5-deoxypentose sugars, such as 5'-methylthioadenosine (MTA) and 5'-deoxyadenosine (5dAdo), into DHAP and an aldehyde species depending on the substrate. Previous work revealed that Escherichia coli strains with the DHAP shunt can utilize exogenous MTA, 5dAdo, and derivatives thereof as sole carbon and energy sources for growth. However, if and how the DHAP shunt was regulated for 5'-deoxynucleoside and 5-deoxypentose sugar metabolism remained unknown. In the present work, the DHAP shunt genes (mtnK, mtnA, and ald2) and a putative transporter gene of E. coli ATCC 25922 are observed to form an operon, which can be expressed from two separat

SUBMITTER: Huening KA 

PROVIDER: S-EPMC12632272 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature