The response regulator BqsR/CarR controls ferrous iron (Fe²⁺) acquisition in <i>Pseudomonas aeruginosa</i>.

ABSTRACT: Pseudomonas aeruginosa is a ubiquitous, Gram-negative bacterium that forms biofilms and is responsible for antibiotic-resistant nosocomial infections in humans. The P. aeruginosa BqsRS two-component system regulates biofilm formation and dispersal by sensing extracytoplasmic Fe²⁺, but the mechanistic details of this process are poorly understood. In this work, we report the crystal and solution structures of the PaBqsR response regulator receiver domain, comprising a (βα)5 response regulator assembly, and the DNA-binding domain, comprising a helix-turn-helix motif. Consistent with its cognate stimulus being Fe²⁺, we show that PaBqsR binds directly to the promoter region of the feo operon that encodes the bacterial Fe²⁺ tra

SUBMITTER: Paredes A 

PROVIDER: S-EPMC12632288 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature