A microfluidics-based platform enables discovery of gametogenic rejuvenation factors in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: During mitotic growth, Saccharomyces cerevisiae cells age by dividing asymmetrically producing young daughter cells while retaining age-associated damage in the mother cell, which will eventually become senescent. Gametogenesis naturally and completely resets this replicative lifespan, even in replicative aged cells. Methods to quantify replicative lifespan resetting have been limited to low-throughput and labor-intensive approaches based on repeated manual micromanipulation of individual cells. Here, we introduce a high-throughput microfluidic-based assay that allows systematic characterization of factors required for gametogenic rejuvenation in S. cerevisiae. A key innovation of this method involves two inert genetic modifications that enable specific enrichment of gametes.

SUBMITTER: Spiri S 

PROVIDER: S-EPMC12632322 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature