Long Term Culture of Germ-Free Zebrafish Using Gamma-Irradiated Feeds.

ABSTRACT: Host associated microbiota play essential roles in regulating digestion, nutrient acquisition, immunity, and xenobiotic metabolism. Disruption of these communities is linked to numerous diseases and health defects though causal mechanisms underpinning these associations remain unclear in most cases. Gnotobiotic zebrafish provide a scalable low-cost in vivo model that is increasingly used to resolve causality in host-microbiota interactions. However, reliance on live diets limits the use of gnotobiotic zebrafish to early life stages where body systems and microbial communities are incompletely developed. As a result, many important host-microbiota interactions may be unable to be studied in this model system. Here we tested a simple method for long-term husbandry of gnotobiotic zebra

SUBMITTER: Okyere L 

PROVIDER: S-EPMC12632343 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature