A Fkh1/2 binding site array in the <i>WHI5</i> promoter drives sub-scaling transcription.

ABSTRACT: Cells typically regulate their size within a relatively tight range by coupling growth to the cell division cycle using a dedicated set of molecular mechanisms. In budding yeast, cells are born with a similar amount of the G1/S inhibitor protein Whi5 that is then diluted by growth throughout G1. As cells grow, Whi5 concentration decreases and cells become more likely to enter the cell cycle. Cells are born in G1 with similar amounts of Whi5 because of the size-independent (sub-scaling) expression of WHI5 mRNA during S/G2/M phases and the equal partitioning of Whi5 protein at division. While the latter is known to be achieved by association with chromatin before anaphase, the mechanism for the former is poorly understood. Through systematic mutations of the WHI5 promoter, we d

SUBMITTER: Kim J 

PROVIDER: S-EPMC12632407 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature