Photoaffinity labeling of protein targets in a complex metazoan: proof-of-concept using a probe for <i>Schistosoma mansoni</i> tubulin.

ABSTRACT: Treatment of schistosomiasis, a prevalent neglected tropical disease, relies precariously on a single drug. The discovery and development of alternative anti-schistosomal small molecules most often relies on phenotypic (whole-organism) screening, whereas target- or "protein-first"-based discovery options are hampered by a paucity of genetic interrogation strategies and a complex biology. Here, we demonstrate the application of photoaffinity labeling (PAL) as a chemical biology strategy to probe protein target-ligand interactions in the schistosome. Using a triazolopyrimidine (TPD) probe that binds tubulin, we established a PAL workflow with living Schistosoma mansoni worms. The probe elicited deleterious phenotypic responses consistent with the TPD series and, upon UV light activati

SUBMITTER: Lucero B 

PROVIDER: S-EPMC12632543 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature