Analysis <i>in vivo</i> using a new method, ARGO (Analysis of Red Green Offset), reveals complexity and cell-type specificity in presynaptic turnover of synaptic vesicle protein Synaptogyrin/SNG-1.

ABSTRACT: In long-lived cells such as neurons, proteostasis involves the regulated degradation and replacement of proteins to ensure their quality and appropriate abundance. Synaptic vesicle (SV) protein turnover in neurons is important for controlling the SV pool size to maintain appropriate levels of neurotransmission; yet, it is incompletely understood, partly due to limited tools for quantifying protein turnover in vivo. We present ARGO (Analysis of Red-Green Offset), a fully genetically encoded, ratiometric fluorescence imaging method that visualizes and quantifies protein turnover with subcellular resolution in vivo. ARGO is inexpensive, modular, and scalable for use in genetically tractable experimental organisms. Using ARGO, we examine the turnover of Synaptogyrin/SNG-1, an evolutionarily co

SUBMITTER: Shiliaev N 

PROVIDER: S-EPMC12632557 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature