Reconstructing EBV reactivation and DNA damage response kinetics in morphologic pseudotime.

ABSTRACT: Epstein-Barr virus (EBV) lytic infection contributes to virus-associated cancers and autoimmunity and depends on viral subversion of host DNA damage responses (DDR). Here, we use high-content screening (HCS) for morphologic and pseudotemporal profiling of single-cell EBV reactivation and DDR dynamics spanning seven lytic induction treatments in nine B cell lymphoma models. We generated an atlas (>750,000 cells) of spatiotemporally distinct phenotypes of immediate-early (IE) and late lytic proteins, viral and cellular DNA replication, and double-stranded break (DSB) DDR factors. Single-cell segmentation, feature extraction, and clustering identify treatment- and model-dependent cell responses and lytic induction. Notably, genotoxin-induced DDR profiles differ in lytic versus latent cells, a

SUBMITTER: Tekle DG 

PROVIDER: S-EPMC12632618 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature