Effects of Non-Cardiac/Non-neurologic Surgery and Anaesthesia on the CSF Proteome, and Modulation by the APOE Mimetic Peptide CN-105.

ABSTRACT:

Objective

To evaluate the effect of non-cardiac/non-neurologic surgery on the CSF proteome, and the effect of the APOE mimetic peptide CN-105 on postoperative CSF proteome changes.

Methods

We performed mass spectrometry-based proteomics on preoperative and 24 hour postoperative cerebrospinal fluid (CSF) samples from 137 patients (age>60) in the MARBLE trial (ct.gov identifier: NCT03802396), who were randomized to receive APOE mimetic peptide CN-105 (or placebo), and in an independent replication cohort. Linear regression was used to evaluate postoperative changes in CSF protein levels and pathway scores (measured by single-sample gene set enrichment analysis [ssGSEA]), with false discovery rate (FDR)-based multiple comparison correction.

Results

Among 2,086 proteins,

SUBMITTER: Zou H 

PROVIDER: S-EPMC12632645 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature