Ex vivo hypothermic oxygenated perfusion allows extended heart preservation in a donation-after-circulatory-death porcine model.

ABSTRACT:

Objectives

Donation after circulatory death (DCD) expands the donor heart pool but is limited by warm ischemia and short preservation times. Hypothermic oxygenated perfusion (HOPE) extends storage beyond the 4-6-hour limit of static cold storage (SCS), yet cellular and molecular responses remain undefined. We evaluate cardiomyocyte integrity and functional recovery of DCD porcine hearts after in situ reanimation with normothermic regional perfusion (NRP) and preservation by SCS (2h) or HOPE (24h) and assess the impact of NRP under DCD conditions.

Methods

Nine Yorkshire pigs underwent DCD cardiectomy. Six animals experienced 15 min warm ischemia followed by 60 min NRP. Three hearts were preserved for 2h with SCS and three for 24h with HOPE. A second DCD group (n=3) underwent

SUBMITTER: Moroi MK 

PROVIDER: S-EPMC12632651 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature