CED-3 caspase promotes dismantling but not onset of non-apoptotic linker cell death in <i>C. elegans</i>.

ABSTRACT: Nuclear degradation accompanies cell death. To study this process, we followed nuclear dismantling of the C. elegans linker cell, which undergoes a non-apoptotic morphologically-conserved death program characterized by nuclear envelope crenellations and cell splitting. We show that although linker cell death is cell autonomous, nucleus elimination follows engulfment and is blocked in rab-35 and arf-6 phagosome maturation mutants. Surprisingly, although linker cell death is independent of the apoptotic caspase CED-3, CED-3 is partially required within the linker cell, and upstream of RAB-35 and ARF-6, for cell splitting, engulfment, and nucleus elimination. In parallel studies, we found that the kinase inhibitor staurosporine causes mouse embryonic fibroblasts to underg

SUBMITTER: Yarychkivska O 

PROVIDER: S-EPMC12632748 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature