Molecular Architecture of the human Citrate Synthase-Malate Dehydrogenase 2 metabolon.

ABSTRACT: Metabolons are transient biomolecular complexes that enhance the efficiency of metabolic pathways through substrate channeling. These complexes are difficult to study because of the transient nature, thus limiting our understanding of how they are formed and regulated. The citric acid cycle is proposed to contain many such complexes although few have been characterized structurally. Here, we provide direct structural evidence for the complex of human Citrate Synthase and human mitochondrial Malate Dehydrogenase 2, which is part of the larger proposed citric acid cycle metabolon. Our structural model supports previous crosslinking studies and suggests that hMDH2 can interact with each subunit of the hCS dimer, forming up to a hexameric complex. However, this complex appears transient as tit

SUBMITTER: Kayll AJ 

PROVIDER: S-EPMC12632796 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature