Tubulin autoregulation factors SCAPER and TTC5 recruit γ-tubulin to non-centrosomal MTOCs for neuronal microtubule nucleation and axon regeneration.

ABSTRACT: Neuronal function and survival depend on highly stereotyped non-centrosomal microtubule (MT) arrays. How these arrays form remains poorly understood. Here we identified a role for SCAPER and TTC5, two factors previously implicated in tubulin mRNA autoregulation, in controlling neuronal MT content through γ-tubulin-dependent nucleation. In C. elegans neurons, loss of scpr-1, ttc-5, or both reduced MT numbers to a similar degree as depletion of γ-tubulin, the main MT nucleator. Using conditional single-cell degradation alleles and endogenous tagging, we find that γ-tubulin nucleates MTs in the neuronal cell body from endosomal puncta, and that scpr-1 and ttc-5 are required to recruit γ-tubulin to these structures. SCPR-1 is also instructive, as its overexpr

SUBMITTER: Xie Y 

PROVIDER: S-EPMC12632806 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature