Massively parallel reporter assay reveals promoter-, position-, and strand-specific effects in transcription start sites.

ABSTRACT: Massively parallel reporter assays (MPRA) are a high-throughput method of assessing the activity of candidate cis-regulatory sequences, and can be used to detect allelic differences at disease-associated variants. Previous MPRA studies have screened thousands of functional SNPs associated with various complex traits and conditions. Most MPRA libraries utilize a single plasmid configuration, a single minimal promoter to drive expression, and a single-strand orientation, which may fail to capture the context-dependent activity of disease-associated cis-regulatory elements. We interrogate the potential regulatory differences introduced by variable MPRA plasmid promoters and positions. We used an MPRA library to quantify the activity of 1,305 pancreatic islet-derived transcription start sites

SUBMITTER: Bose M 

PROVIDER: S-EPMC12632999 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature