Urinary extracellular vesicles for high-precision bladder cancer subtyping and prognosis.

ABSTRACT: Bladder cancer exhibits molecular heterogeneity that complicates early diagnosis and prognosis, and drives confounding clinical outcomes. Non-muscle invasive and muscle-invasive subtypes, especially for intermediate to high grade, carry a 25 - 50% progression-free survival rate, underscoring the need for high precision prognostic strategy. Urinary extracellular vesicles (uEVs) are promising carriers of tumor-derived RNAs and proteins. However, significant challenges in studying uEVs arise from the diverse cellular origin of uEVs associated with the dynamic composition of urine, which presents roadblocks for developing the clinical utility of uEVs. We introduced an AI-driven EV liquid biopsy pipeline that integrates (1) standardized EV isolation via NanoPom magnetic beads, (2) transcriptomi

SUBMITTER: Greenberg ZF 

PROVIDER: S-EPMC12633135 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature