Comparative gene editing reduces dopamine receptor levels across rodent species.

ABSTRACT: Translational challenges in neuroscience originate from species-specific differences that limit the generalizability of experimental findings. Comparative approaches can help distinguish conserved from species-specific mechanisms, but their application has been limited by the lack of molecular tools beyond traditional model organisms, complicating direct comparisons of conserved and divergent mechanisms of neural function. This gap is particularly evident for the dopaminergic system, a key regulator of motivated behaviors across species and the principal pharmacological target for current psychotherapies. Building on our recent development of comparative gene editing, we here present an adeno-associated virus-mediated CRISPR/Cas9 strategy to reduce in vivo dopamine receptors D1 and

SUBMITTER: Karkare SC 

PROVIDER: S-EPMC12633220 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature