A scalable filtration-based method for isolating exomeres and other nanoscale extracellular particles.

ABSTRACT: Extracellular particles, including extracellular vesicles (EVs) and non-vesicular extracellular particles (NVEPs), enable intercellular communication by transferring regulatory miRNAs and other biomolecules. While EVs have been studied for drug delivery, NVEPs remain relatively unexplored. Exomeres, a recently discovered class of NVEPs enriched in RNAi proteins, preferentially carry miRNAs and deliver them to cells more effectively than EVs, underscoring their potential as vehicles for therapeutic RNAs. One current limitation to studying and applying exomeres for therapeutic RNA delivery is the shortage of scalable, cost-effective, and rapid isolation methods. Here, we investigated whether tangential flow filtration (TFF), a common bioseparation approach that separates species by size, wou

SUBMITTER: Scarborough T 

PROVIDER: S-EPMC12633226 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature