Genetic dissection of transcription start site selection by RNA Polymerase II in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Transcription initiation by RNA Pol II is driven by a preinitiation complex (PIC) comprising Pol II and general transcription factors (GTFs): TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH. In Saccharomyces cerevisiae, transcription start site (TSS) selection proceeds by a promoter scanning mechanism where the PIC scans downstream for TSSs. To determine what factors participate in TSS selection by promoter scanning we designed and implemented forward genetic selections using initiation-sensitive genetic reporters. These reporters were of two types, either being sensitive to upstream or downstream TSS shifts, allowing us to detect alleles that affect promoter scanning in different directions. From >1000 candidates, we identified three primary classes of mutants: existing and novel mutan

SUBMITTER: Arora P 

PROVIDER: S-EPMC12633254 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature