Multi-site Assessment of Methods for Cell Preservation Upstream of Single Cell RNA Sequencing.

ABSTRACT: Single cell RNA sequencing (scRNA-seq) is a revolutionary technique to identify cell types and their molecular phenotype in heterogeneous biological specimens. ScRNA-seq typically requires fresh, high quality single cell suspensions that are processed immediately to preserve their molecular profiles. This presents a challenge for samples with long preparation times and prevents collection at remote sites lacking the required instrumentation for sample processing. Recently, several commercial assays have been released that enable sample preservation at the time of collection either via fixation or cryopreservation, allowing for sample processing to occur months after the initial collection. The Association of Biomolecular Research Facilities' (ABRF) DNA Sequencing (DSRG) and Genomics Bioinf

SUBMITTER: Kolling FW 

PROVIDER: S-EPMC12633272 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature