Interregional human assembloids recapitulate fetal brain morphologies and enhance neuronal complexity.

ABSTRACT: Neuronal morphology governs how neurons connect, integrate, and process information, offering critical insights into the functional architecture of the brain. Characterizing the three-dimensional (3D) morphology of individual neurons is key not only for mapping circuit connectivity but also for understanding the cellular diversity that emerges during development. Neural organoids are valuable models of human brain development and disease, yet their morphological complexity remains poorly characterized despite advances in single-cell transcriptomics. Here, we use 3D confocal imaging and manual reconstruction of 735 neurons to analyze forebrain (dorsal and ventral) and thalamic (dorsal and ventral) organoids, as well as forebrain, thalamic, and corticothalamic assembloids. We find that organ

SUBMITTER: Antunes ASLM 

PROVIDER: S-EPMC12633276 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature