"A high throughput flow cytometry assay for quantifying type 3 secretion system assembly in <i>Salmonella</i>".

ABSTRACT: Recombinant protein production in bacteria is limited by costly cell lysis and multi-step purification. To bypass these limitations, leveraging native bacterial secretion systems to secrete proteins offers a promising alternative. The type 3 secretion system (T3SS) of Salmonella enterica serovar Typhimurium can secrete proteins at hundreds of milligrams per liter. While promising, higher titers from this and other natural systems are needed to be relevant commercially. A major engineering target to enhance protein secretion is the T3SS secretion apparatus. However, efforts to quantify its assembly are hindered by bottlenecks in scalability and complexity. Assembly involves ~20 structural proteins forming with precise stoichiometry under dynamic regulation. Membrane- and periplasmic-

SUBMITTER: Summers JS 

PROVIDER: S-EPMC12633367 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature