ADAPT-M: A workflow for rapid, quantitative <i>in vitro</i> measurements of enriched protein libraries.

ABSTRACT: Protein-protein interactions underpin most cellular interactions, and engineered binders present powerful tools for probing biology and developing novel therapeutics. One bottleneck in binder generation is the scalable, quantitative characterization of these interactions. We present ADAPT-M (Affinity Determination by Adaptation of ProTein binders for Microfluidics), a streamlined workflow that connects yeast surface display (YSD) with in vitro affinity and kinetic measurements using the high-throughput STAMMPPING microfluidic platform. ADAPT-M quantifies K _ds and dissociation kinetic parameters for hundreds of enriched protein variants in under one week without requiring hands-on protein purification. We applied ADAPT-M to

SUBMITTER: Perez CP 

PROVIDER: S-EPMC12633381 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature