Investigating Overflow Metabolism in Heterotrophic Cultures of the Green Alga <i>Chromochloris zofingiensis</i>.

ABSTRACT: Chromochloris zofingiensis is of interest for its ability to perform a reversible trophic switch in the presence of glucose that is characterized by a shutdown of photosynthesis and an accumulation of energy storage metabolites. Previous work has shown that this trophic switch is accompanied by overflow metabolism and the production of lactate in aerobic conditions. This trophic switch is not observed in nutrient replete media. We utilized isotopically assisted metabolic flux analysis to characterize intracellular flux distributions that are associated with different metabolic phenotypes observed in this organism in different media formulations in light and dark conditions. The results of this analysis showed differences in flux through carbon fixation reactions, the TCA cycle and t

SUBMITTER: Meagher M 

PROVIDER: S-EPMC12633386 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature